Dielectrophoretic Separation of Mouse Melanoma Clones

Ahmet C. Sabuncu; Jie A. Liu; Stephen J. Beebe; Ali Beskok; Alan Z Liu

doi:10.1063/1.3447702

Dielectrophoretic Separation of Mouse Melanoma Clones

Ahmet C. Sabuncu, Jie A. Liu, Stephen J. Beebe, Ali Beskok, Alan Z Liu

Old Dominion University

Research output: Contribution to journal › Article › peer-review

Abstract

Dielectrophoresis (DEP) is employed to differentiate clones of mouse melanoma B16F10 cells. Five clones were tested on microelectrodes. At a specific excitation frequency, clone 1 showed a different DEP response than the other four. Growth rate, melanin content, recovery from cryopreservation, and in vitro invasive studies were performed. Clone 1 is shown to have significantly different melanin content and recovery rate from cryopreservation. This paper reports the ability of DEP to differentiate between two malignant cells of the same origin. Different DEP responses of the two clones could be linked to their melanin content.

Original language	American English
Journal	Biomicrofluidics
Volume	4
DOIs	https://doi.org/10.1063/1.3447702
State	Published - Jun 1 2010

Keywords

Dielectrophoresis
Melanoma
Cloning
Microelectrodes
Cryopreservation

Disciplines

Biomedical Engineering and Bioengineering

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10.1063/1.3447702License: CC BY

Dielectrophoretic Separation of Mouse Melanoma ClonesFinal published version, 373 KBLicense: CC BY

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title = "Dielectrophoretic Separation of Mouse Melanoma Clones",

abstract = " Dielectrophoresis (DEP) is employed to differentiate clones of mouse melanoma B16F10 cells. Five clones were tested on microelectrodes. At a specific excitation frequency, clone 1 showed a different DEP response than the other four. Growth rate, melanin content, recovery from cryopreservation, and in vitro invasive studies were performed. Clone 1 is shown to have significantly different melanin content and recovery rate from cryopreservation. This paper reports the ability of DEP to differentiate between two malignant cells of the same origin. Different DEP responses of the two clones could be linked to their melanin content.",

keywords = "Dielectrophoresis, Melanoma, Cloning, Microelectrodes, Cryopreservation",

author = "Sabuncu, {Ahmet C.} and Liu, {Jie A.} and Beebe, {Stephen J.} and Ali Beskok and Liu, {Alan Z}",

note = "pSabuncu, A.C., Liu, J.A., Beebe, S.J., Beskok, A. (2010). Dielectrophoretic separation of mouse melanoma clones. emBiomicrofluidics, 4/em(2), 021101. doi: 10.1063/1.3447702/p",

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T1 - Dielectrophoretic Separation of Mouse Melanoma Clones

AU - Sabuncu, Ahmet C.

AU - Liu, Jie A.

AU - Beebe, Stephen J.

AU - Beskok, Ali

AU - Liu, Alan Z

N1 - pSabuncu, A.C., Liu, J.A., Beebe, S.J., Beskok, A. (2010). Dielectrophoretic separation of mouse melanoma clones. emBiomicrofluidics, 4/em(2), 021101. doi: 10.1063/1.3447702/p

PY - 2010/6/1

Y1 - 2010/6/1

N2 - Dielectrophoresis (DEP) is employed to differentiate clones of mouse melanoma B16F10 cells. Five clones were tested on microelectrodes. At a specific excitation frequency, clone 1 showed a different DEP response than the other four. Growth rate, melanin content, recovery from cryopreservation, and in vitro invasive studies were performed. Clone 1 is shown to have significantly different melanin content and recovery rate from cryopreservation. This paper reports the ability of DEP to differentiate between two malignant cells of the same origin. Different DEP responses of the two clones could be linked to their melanin content.

AB - Dielectrophoresis (DEP) is employed to differentiate clones of mouse melanoma B16F10 cells. Five clones were tested on microelectrodes. At a specific excitation frequency, clone 1 showed a different DEP response than the other four. Growth rate, melanin content, recovery from cryopreservation, and in vitro invasive studies were performed. Clone 1 is shown to have significantly different melanin content and recovery rate from cryopreservation. This paper reports the ability of DEP to differentiate between two malignant cells of the same origin. Different DEP responses of the two clones could be linked to their melanin content.

KW - Dielectrophoresis

KW - Melanoma

KW - Cloning

KW - Microelectrodes

KW - Cryopreservation

UR - https://digitalcommons.odu.edu/bioelectrics_pubs/45

U2 - 10.1063/1.3447702

DO - 10.1063/1.3447702

M3 - Article

SN - 1932-1058

VL - 4

JO - Biomicrofluidics

JF - Biomicrofluidics

ER -

Dielectrophoretic Separation of Mouse Melanoma Clones

Abstract

Keywords

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