Disinfection of B. Subtilis Cells in Suspension using Ultraviolet Light Emitting Diodes (LEDS) in the Presence of TiO2

Dennis W. Province; Shannon O'Neil; Keri Higgins; Paul J. Smith; Kristin Dooley; Joey Curtis; Adam M. Grippo; John W. Rino; Susan D. Allen

doi:10.1063/1.3587474

Disinfection of B. Subtilis Cells in Suspension using Ultraviolet Light Emitting Diodes (LEDS) in the Presence of TiO2

Dennis W. Province, Shannon O'Neil, Keri Higgins, Paul J. Smith, Kristin Dooley, Joey Curtis, Adam M. Grippo, John W. Rino, Susan D. Allen

Arkansas State University - Main Campus

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The concentration of vegetative Bacillus subtilis (B. subtilis) in phosphate buffered saline decreased when subjected to ultraviolet (UV) light from light emitting diodes (LEDs) in the presence of 0.01% Degussa P25 titanium dioxide (TiO2) as compared to a sample that contained bacteria only, a sample of bacteria that contained 0.01% TiO2, and bacteria that was subjected to the same UV light but no TiO2. The starting concentration of each sample was on the order of 104104 colony forming units per milliliter (CFU/mL) and the time required for complete kill was less than 100min when the peak wavelength was 370 nm for the four LED light source at a total LED power of 0.8 milliWatts (mW), decreasing to less than 75 min for a total LED power of 3 mW. Changing the peak wavelength by 7 nm to 377 nm decreased the kill of vegetative B. subtilis to less than 1 log at 100 min for 5 mW total LED power.

Original language	American English
Title of host publication	AIP Conference Proceedings
DOIs	https://doi.org/10.1063/1.3587474
State	Published - Jun 2011
Externally published	Yes

Keywords

light emitting diodes
bacteria
ultraviolet light
cell cultures
suspensions

Disciplines

Bacteria
Biological and Chemical Physics

Access to Document

10.1063/1.3587474License: Unspecified

Cite this

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title = "Disinfection of B. Subtilis Cells in Suspension using Ultraviolet Light Emitting Diodes (LEDS) in the Presence of TiO2",

abstract = " The concentration of vegetative Bacillus subtilis (B. subtilis) in phosphate buffered saline decreased when subjected to ultraviolet (UV) light from light emitting diodes (LEDs) in the presence of 0.01% Degussa P25 titanium dioxide (TiO2) as compared to a sample that contained bacteria only, a sample of bacteria that contained 0.01% TiO2, and bacteria that was subjected to the same UV light but no TiO2. The starting concentration of each sample was on the order of 104104 colony forming units per milliliter (CFU/mL) and the time required for complete kill was less than 100min when the peak wavelength was 370 nm for the four LED light source at a total LED power of 0.8 milliWatts (mW), decreasing to less than 75 min for a total LED power of 3 mW. Changing the peak wavelength by 7 nm to 377 nm decreased the kill of vegetative B. subtilis to less than 1 log at 100 min for 5 mW total LED power.",

keywords = "light emitting diodes, bacteria, ultraviolet light, cell cultures, suspensions",

author = "Province, {Dennis W.} and Shannon O'Neil and Keri Higgins and Smith, {Paul J.} and Kristin Dooley and Joey Curtis and Grippo, {Adam M.} and Rino, {John W.} and Allen, {Susan D.}",

year = "2011",

month = jun,

doi = "10.1063/1.3587474",

language = "American English",

booktitle = "AIP Conference Proceedings",

}

TY - CHAP

T1 - Disinfection of B. Subtilis Cells in Suspension using Ultraviolet Light Emitting Diodes (LEDS) in the Presence of TiO2

AU - Province, Dennis W.

AU - O'Neil, Shannon

AU - Higgins, Keri

AU - Smith, Paul J.

AU - Dooley, Kristin

AU - Curtis, Joey

AU - Grippo, Adam M.

AU - Rino, John W.

AU - Allen, Susan D.

PY - 2011/6

Y1 - 2011/6

N2 - The concentration of vegetative Bacillus subtilis (B. subtilis) in phosphate buffered saline decreased when subjected to ultraviolet (UV) light from light emitting diodes (LEDs) in the presence of 0.01% Degussa P25 titanium dioxide (TiO2) as compared to a sample that contained bacteria only, a sample of bacteria that contained 0.01% TiO2, and bacteria that was subjected to the same UV light but no TiO2. The starting concentration of each sample was on the order of 104104 colony forming units per milliliter (CFU/mL) and the time required for complete kill was less than 100min when the peak wavelength was 370 nm for the four LED light source at a total LED power of 0.8 milliWatts (mW), decreasing to less than 75 min for a total LED power of 3 mW. Changing the peak wavelength by 7 nm to 377 nm decreased the kill of vegetative B. subtilis to less than 1 log at 100 min for 5 mW total LED power.

AB - The concentration of vegetative Bacillus subtilis (B. subtilis) in phosphate buffered saline decreased when subjected to ultraviolet (UV) light from light emitting diodes (LEDs) in the presence of 0.01% Degussa P25 titanium dioxide (TiO2) as compared to a sample that contained bacteria only, a sample of bacteria that contained 0.01% TiO2, and bacteria that was subjected to the same UV light but no TiO2. The starting concentration of each sample was on the order of 104104 colony forming units per milliliter (CFU/mL) and the time required for complete kill was less than 100min when the peak wavelength was 370 nm for the four LED light source at a total LED power of 0.8 milliWatts (mW), decreasing to less than 75 min for a total LED power of 3 mW. Changing the peak wavelength by 7 nm to 377 nm decreased the kill of vegetative B. subtilis to less than 1 log at 100 min for 5 mW total LED power.

KW - light emitting diodes

KW - bacteria

KW - ultraviolet light

KW - cell cultures

KW - suspensions

U2 - 10.1063/1.3587474

DO - 10.1063/1.3587474

M3 - Chapter

BT - AIP Conference Proceedings

ER -