Live cell measurements of interaction forces and binding kinetics between Discoidin Domain Receptor 1 (DDR1) and collagen I with atomic force microscopy

Peter M. Hoffmann; Anwesha Sarka; Anjum Sohail; Jiayin Dong; Marco Prunotto; Kazuhiko Shinki; Rafael Fridman

doi:10.1016/j.bbagen.2019.07.011

Live cell measurements of interaction forces and binding kinetics between Discoidin Domain Receptor 1 (DDR1) and collagen I with atomic force microscopy

Peter M. Hoffmann
, Anwesha Sarka
, Anjum Sohail
, Jiayin Dong
, Marco Prunotto
, Kazuhiko Shinki
, Rafael Fridman

Research output: Contribution to journal › Article › peer-review

Abstract

Background

Discoidin Domain Receptors (DDRs) are membrane-tethered proteins of the receptor tyrosine kinase family, which signal in response to collagen. DDR expression is associated with human diseases, including fibrosis and cancer. The role of DDRs in human pathogenesis is mediated by dysregulated receptor function in response to the collagenous milieu. Thus, understanding DDR-collagen interactions is important for developing novel therapeutic strategies against DDRs.

Original language	American English
Journal	BBA-General Subjects
DOIs	https://doi.org/10.1016/j.bbagen.2019.07.011
State	Published - 2019

Disciplines

Chemistry

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@article{5a4f394bab8e4da09238f3f1e2d98551,

title = "Live cell measurements of interaction forces and binding kinetics between Discoidin Domain Receptor 1 (DDR1) and collagen I with atomic force microscopy",

abstract = " Background Discoidin Domain Receptors (DDRs) are membrane-tethered proteins of the receptor tyrosine kinase family, which signal in response to collagen. DDR expression is associated with human diseases, including fibrosis and cancer. The role of DDRs in human pathogenesis is mediated by dysregulated receptor function in response to the collagenous milieu. Thus, understanding DDR-collagen interactions is important for developing novel therapeutic strategies against DDRs.",

author = "Hoffmann, \{Peter M.\} and Anwesha Sarka and Anjum Sohail and Jiayin Dong and Marco Prunotto and Kazuhiko Shinki and Rafael Fridman",

note = "The new approach is applicable to other receptor-ligand systems and allows the determination of critical parameters at the single cell/single molecule level - in particular, the direct determination of kinetic and density differences of receptors in different cell types. This capability should prove ...",

year = "2019",

doi = "10.1016/j.bbagen.2019.07.011",

language = "American English",

journal = "BBA-General Subjects",

issn = "1872-8006",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Live cell measurements of interaction forces and binding kinetics between Discoidin Domain Receptor 1 (DDR1) and collagen I with atomic force microscopy

AU - Hoffmann, Peter M.

AU - Sarka, Anwesha

AU - Sohail, Anjum

AU - Dong, Jiayin

AU - Prunotto, Marco

AU - Shinki, Kazuhiko

AU - Fridman, Rafael

N1 - The new approach is applicable to other receptor-ligand systems and allows the determination of critical parameters at the single cell/single molecule level - in particular, the direct determination of kinetic and density differences of receptors in different cell types. This capability should prove ...

PY - 2019

Y1 - 2019

N2 - Background Discoidin Domain Receptors (DDRs) are membrane-tethered proteins of the receptor tyrosine kinase family, which signal in response to collagen. DDR expression is associated with human diseases, including fibrosis and cancer. The role of DDRs in human pathogenesis is mediated by dysregulated receptor function in response to the collagenous milieu. Thus, understanding DDR-collagen interactions is important for developing novel therapeutic strategies against DDRs.

AB - Background Discoidin Domain Receptors (DDRs) are membrane-tethered proteins of the receptor tyrosine kinase family, which signal in response to collagen. DDR expression is associated with human diseases, including fibrosis and cancer. The role of DDRs in human pathogenesis is mediated by dysregulated receptor function in response to the collagenous milieu. Thus, understanding DDR-collagen interactions is important for developing novel therapeutic strategies against DDRs.

UR - https://pubmed.ncbi.nlm.nih.gov/31351093/

U2 - 10.1016/j.bbagen.2019.07.011

DO - 10.1016/j.bbagen.2019.07.011

M3 - Article

SN - 1872-8006

JO - BBA-General Subjects

JF - BBA-General Subjects

ER -

Live cell measurements of interaction forces and binding kinetics between Discoidin Domain Receptor 1 (DDR1) and collagen I with atomic force microscopy

Abstract

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